HEK293T cells were infected with ASFV-P31 at an MOI of 1 for the co-IP assay. At 48 hpi, the cells were lysed with NP-40 for 30 min at 4°C and centrifuged for 10 min at 4°C; the cellular supernatants were incubated with an irrelevant isotype antibody IgG (catalog no. A7016; Beyotime) serving as a control and anti-H240R PAb with protein G plus agarose (catalog no. IP10-10ML; Merck-Millipore) at 4°C for 12 h. The agarose was washed six times with phosphate-buffered saline (PBS), and the bound proteins were subjected to Western blotting (49 (link)).
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