Culture medium was refreshed after 1, 24 and subsequently every 48 h by replacing 1.6 of 2.4 mL in the BMCC. Preload was readjusted to 1 mN at every medium exchange. Isoprenaline was added after each medium exchange, maintaining the final concentration constant at 1 µM. Without isoprenaline enhancement, LMS contraction force quenched to a minimum level. Contraction force was measured using dedicated MyoDish Software3 (link).
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