Isoprenaline-Induced LMS Contraction
Corresponding Organization :
Other organizations : Erasmus MC
Variable analysis
- Culture medium refreshment frequency (1, 24, and subsequently every 48 h)
- Isoprenaline addition after each medium exchange (maintaining a final concentration of 1 µM)
- Contraction force of the LMS
- Volume of medium replaced (1.6 of 2.4 mL in the BMCC)
- Preload readjusted to 1 mN at every medium exchange
- Not mentioned
- Without isoprenaline enhancement, LMS contraction force quenched to a minimum level
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