Difference between the means of two groups and more than two groups were calculated using the Student’s t test and analysis of variance (ANOVA) statistical analysis, respectively. ANOVA significant results were followed by the post-hoc Tukey’s test. Data are presented as mean ± SD unless otherwise indicated. N = number of independent experiments or animals. In myograph experiments, relaxation (%) by acetylcholine was calculated as the reversal of tension induced by the contractile agonist (phenylephrine). Acetylcholine-induced relaxations were analyzed using 2-way ANOVA with Bonferroni post-hoc test for pairwise comparisons. p <0.05 was considered significant.
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