Routine Bacterial Culturing Techniques

Bacterial strains are listed in Table S1 (available with the online version of this article). Unless otherwise stated, chemicals and reagents, including antibiotics, were purchased from Sigma-Aldrich and components to prepare growth media were purchased from Oxoid. All strains were grown in lysogeny broth (LB) (10 g l⁻¹ NaCl) and agar (1.5 % w/v) [47 ] for routine growth with shaking at 200 r.p.m., as appropriate. E. coli strains were incubated at 37 °C and P. putida strains at 30 °C. Antibiotics were used at (µg ml⁻¹): rifampicin (Rif), 20 for Pseudomonas; tetracycline (Tc), 20 for P. putida and 10 for E. coli; kanamycin (Km), 50 for P. putida and 25 for E. coli; ampicillin (Ap) 100 for E. coli; piperacillin (Pip), 25 for P. putida.