Culturing Expi293F and Drosophila S2 Cells

Expi293F cells (Thermo Fisher Scientific) were maintained in suspension in Expi293 expression medium (Thermo Fisher Scientific) at 37 °C, 8% CO₂, in a shaking incubator at 120 rpm.
Drosophila S2 cells^{42 (link)} were cultured at 25 °C using EX-CELL 420 medium (Sigma-Aldrich) with 100 U/ml penicillin, 0.1 mg/ml streptomycin, and 10% fetal bovine serum at 115 rpm in an Innova 44 shaking incubator.

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Ragotte R.J., Pulido D., Lias A.M., Quinkert D., Alanine D.G., Jamwal A., Davies H., Nacer A., Lowe E.D., Grime G.W., Illingworth J.J., Donat R.F., Garman E.F., Bowyer P.W., Higgins M.K, & Draper S.J. (2022). Heterotypic interactions drive antibody synergy against a malaria vaccine candidate. Nature Communications, 13, 933.

Publication 2022

Expi293F cells Expi293 expression medium EX-CELL 420 medium

Cell Drosophila Fetal bovine serum Penicillin Streptomycin

Corresponding Organization :

Other organizations : University of Oxford, National Institute for Biological Standards and Control, University of Surrey, Jenner Institute

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Variable analysis

independent variables

Culture conditions for Expi293F cells (37 °C, 8% CO2, 120 rpm shaking incubator)
Culture conditions for Drosophila S2 cells (25 °C, 115 rpm shaking incubator, with penicillin, streptomycin, and 10% fetal bovine serum)

dependent variables

Not explicitly mentioned

control variables

Culture medium for Expi293F cells (Expi293 expression medium)
Culture medium for Drosophila S2 cells (EX-CELL 420 medium)

controls

No positive or negative controls were explicitly mentioned

Annotations

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