HER-2/neu (neu-N) transgenic mice were purchased from Jackson Laboratory (FVB-Tg(MMTV-Erbb2)NK1Mul/J), and bred and housed at the Johns Hopkins animal facility. Experiments were performed using female mice between 6 and 12 weeks old and protocols approved by the Animal Care and Use Committee of the Johns Hopkins University School of Medicine. High and low avidity T cell receptor (TCR) transgenic mice were generated from RNEU420-429 specific T cell clones as described (22 (link)). Dilutional tetramer staining was used to verify the avidity of each mouse before use.
Protocol detail
Transgenic Mice for Immunotherapy Research
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Animal
Clones
Dilutional
Erbb2
Female
Her 2 neu
Mmtv
Mouse
T cell
T cell receptor
Tetramer
Transgenic mice
Corresponding Organization : Johns Hopkins University
Top 5 similar protocols
Variable analysis
independent variables
- High and low avidity T cell receptor (TCR) transgenic mice
- Not explicitly mentioned
- Female mice between 6 and 12 weeks old
- Protocols approved by the Animal Care and Use Committee of the Johns Hopkins University School of Medicine
- Positive control: Dilutional tetramer staining was used to verify the avidity of each mouse before use.
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!