Western Blot Analysis of Cell Signaling

Western blots were performed as described [41 (link)]. Primary antibody: anti-PPAR-α (1:1000, Santa Cruz Biotechnology, Heidelberg, Germany, sc-398394), anti-TRL4 (1:1000, Santa Cruz Biotechnology, Heidelberg, Germany, sc-293072), anti-pERK 1/2 (1:1000, Santa Cruz Biotechnology, Heidelberg, Germany, sc-7383), anti-NLRP3 (1:500, Santa Cruz Biotechnology, Heidelberg, Germany, sc-134306), anti-transforming growth factor-beta3 (TGF-β3), (1:1000, Santa Cruz Biotechnology, Heidelberg, Germany, sc-166833), anti-claudin-11 (1:100, Santa Cruz Bio-technology, Heidelberg, Germany, sc-271232), or anti-occludin (1:100, Santa Cruz Biotechnology, Heidelberg, Germany, sc-133255) [44 (link),45 (link)].

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Antonuccio P., Marini H.R., Micali A., Romeo C., Granese R., Retto A., Martino A., Benvenga S., Cuzzocrea S., Impellizzeri D., Di Paola R., Fusco R., Cervellione R.M, & Minutoli L. (2021). The Nutraceutical N-Palmitoylethanolamide (PEA) Reveals Widespread Molecular Effects Unmasking New Therapeutic Targets in Murine Varicocele. Nutrients, 13(3), 734.

Publication 2021

anti-PPAR-α anti-TRL4 anti-pERK 1/2 anti-NLRP3 anti-occludin

Antibody Claudin Occludin Ppar Transforming growth factor beta3 Western blots

Corresponding Organization : University of Messina

Other organizations : Royal Manchester Children's Hospital

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Variable analysis

independent variables

Primary antibody concentrations: 1:1000 for anti-PPAR-α, anti-TRL4, anti-pERK 1/2, and anti-TGF-β3; 1:500 for anti-NLRP3; 1:100 for anti-claudin-11 and anti-occludin

dependent variables

Protein expression levels of PPAR-α, TRL4, pERK 1/2, NLRP3, TGF-β3, claudin-11, and occludin

control variables

Experimental conditions (e.g., sample preparation, electrophoresis, and transfer) as described in the referenced publication [41]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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