Phylogenetic Analysis of C. elegans SNARE Proteins

For this study, about 30 C. elegans SNARE and SNARE-associated protein sequences were retrieved directly from WormBase based on previous annotation (https://wormbase.org) (accessed on 11 May 2022) [28 (link)]. The protein sequences of their closest human orthologs were retrieved from UniProt (https://www.uniprot.org) (accessed on 11 May 2022). Initially, the protein sequences obtained were aligned using Clustal Omega, which also gave a neighbor-joining tree without distance corrections (https://www.ebi.ac.uk/Tools/msa/clustalo/) (accessed on 12 May 2022) [66 (link)]. The phylogenetic tree data obtained from Clustal Omega were then further annotated using iTOL (https://itol.embl.de) (accessed on 8 August 2022). Particularly, the unrooted display mode was selected using the equal-angle algorithm by default [29 (link)].

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Gkikas I., Daskalaki I., Kounakis K., Tavernarakis N, & Lionaki E. (2023). MitoSNARE Assembly and Disassembly Factors Regulate Basal Autophagy and Aging in C. elegans. International Journal of Molecular Sciences, 24(4), 4230.

Publication 2023

Human Protein human Protein sequences Snare Tree

Corresponding Organization : University of Crete

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Variable analysis

independent variables

Protein sequences of C. elegans SNARE and SNARE-associated proteins

dependent variables

Neighbor-joining tree without distance corrections

control variables

The protein sequences of the closest human orthologs of the C. elegans SNARE and SNARE-associated proteins

controls

No positive or negative controls were explicitly specified in the provided information.

Annotations

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