These were performed exactly as described previously (Selyunin and Mukhopadhyay, 2015 (link)). To summarize details of the microscope in this study, we used a sweptfield confocal microscope equipped with a four-line high-power laser launch and a 100× 1.45 NA oil immersion objective (Nikon). An iXon3 X3 DU897 electron-multiplying charge-coupled device camera (Andor Technology) was used for image capture. All images were captured as z stacks with 0.2-µm spacing between individual frames. Images depicted in the figures are maximum-intensity projections of the stacks.
All analyses were performed using ImageJ (National Institutes of Health) as described previously (Selyunin and Mukhopadhyay, 2015 (link)).
All analyses were performed using ImageJ (National Institutes of Health) as described previously (Selyunin and Mukhopadhyay, 2015 (link)).
