SLC44A2 Gene Amplification and Sequencing

The primers (Eurofins MWG Operon; Huntsville, AL) for amplification (Table S1) and sequencing (Table S2) were designed using the Primer3 web resource.^{32 (link)} All 22 exons and their adjacent intronic sequences of the SLC44A2 gene were amplified (DNA Engine Tetrad 2 Peltier Thermal Cycler; Bio-Rad, Hercules, CA) using only 8 reactions (Table S1).

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Chen Q., Srivastava K., Ardinski S.C., Lam K., Huvard M.J., Schmid P, & Flegel W.A. (2015). Full length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen-3 (HNA-3). Transfusion, 56(3), 729-736.

Publication 2015

DNA Engine Tetrad 2 Peltier Thermal Cycler

Exons Gene Intronic Operon Primers

Corresponding Organization : National Institutes of Health Clinical Center

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Variable analysis

independent variables

Primers for amplification and sequencing

dependent variables

Amplification of 22 exons and their adjacent intronic sequences of the SLC44A2 gene

control variables

8 reactions for amplification of all 22 exons and their adjacent intronic sequences of the SLC44A2 gene

controls

No positive or negative controls were explicitly mentioned.

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