Immunoblotting Analysis Protocol

Immunoblotting analyses were performed according to standard protocols (described in [5 (link),17 (link)]). After incubation with appropriate secondary antibody, signals were detected using immunochemiluminescent reagents (GE Healthcare, Piscataway, NJ). Equal protein loading in each lane was confirmed with a β-actin antibody (#A300-491, Bethyl). See Supplementary Methods for detailed protocol.

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Coleman D.J., Chagani S., Hyter S., Sherman A.M., Löhr C.V., Liang X., Ganguli-Indra G, & Indra A.K. (2014). Loss of Keratinocytic RXRα Combined with Activated CDK4 or oncogenic NRAS Generates UVB-induced Melanomas via Loss of p53 and PTEN in the Tumor Microenvironment. Molecular cancer research : MCR, 13(1), 186-196.

Publication 2014

immunochemiluminescent reagents β-actin antibody

Actin Antibody Immunoblotting analyses Protein

Corresponding Organization : Oregon Health & Science University

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Variable analysis

independent variables

Incubation with appropriate secondary antibody

dependent variables

Signals detected using immunochemiluminescent reagents

control variables

Equal protein loading in each lane confirmed with a β-actin antibody

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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