Betalamic acid and the betalains indicaxanthin, dopaxanthin, phenylalanine‐betaxanthin, phenylethylamine‐betaxanthin, indoline carboxylic acid‐betacyanin and indoline‐betacyanin were obtained by a semi‐synthesis method (Gandía‐Herrero et al., 2006, 2010a) and subsequently purified for use as real standard betalains for identification purposes.