All cloning experiments were performed in E. coli DH10B cells (Invitrogen) and cells were grown at 37 °C in Luria-Bertani broth (LB) or on agar plates and supplemented with 200 μg/mL hygromycin B (Euromedex). The M. smegmatis strain mc2155 GroEL1ΔC54 (link) used for expression experiments was grown at 37 °C under stirring (220 rpm) in Middlebrook 7H9 broth (Difco) supplemented with 0.05% Tween80 (v/v), 0.2% Glycerol (v/v), 0.5% BSA (w/v), 0.2% Glucose and 150 mM NaCl or on Middlebrook 7H11 agar. hygromycin B at 50 μg/mL was used for the selection of transformed bacteria.
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