Culturing Human and Mouse Keratinocyte Cell Lines

The human keratinocyte cell line HaCaT was obtained from the China Center for Type Culture Collection (0106) and the mouse keratinocyte cell line PAM212 was purchased from the Mingzhoubio (MZ-2610). HaCaT and PAM212 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, C11995500BT) and Roswell Park Memorial Institute 1640 medium (RPMI-1640; Gibco, 11875119), respectively. And both mediums were supplemented with 10% (v:v) fetal bovine serum (FBS; Thermo Fisher Scientific, 10099141), 100 U/mL penicillin G, and 0.1 mg/mL streptomycin sulfate (Thermo Fisher Scientific, 15140122). The cell culture conditions were 37 °C and 5% CO₂. All cells were found to be free from mycoplasma contamination.