CN activity in vitro was determined according to the instructions of recombinant/purified CN activity kits (Abcam). Briefly, after calcineurin and calcineurin assay buffer were mixed, different concentrations of tutin are added for 5 min. The calcineurin substrate was added to start the reaction. This assay was then terminated by thegreen assay reagent (100 μL). The absorbance of OD620 was detected and the CN activity was calculated.
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