The last stage of the current study was the codon optimization for the designed potential vaccine where we employed the JCAT server for this purpose (Grote et al., 2005 (link)). Here, we selected E. coli k-12 strain as the expression organism as it is frequently used in gene cloning experiments (the first stage for wet lab validation of the current potential vaccine). The codon adaptation index (CAI), a value that is calculated by the server, gives an estimation for the constructed potential vaccine to be expressed in E. coli k-12.
Gouda A.M., Soltan M.A., Abd-Elghany K., Sileem A.E., Elnahas H.M., Ateya M.A., Elbatreek M.H., Darwish K.M., Bogari H.A., Lashkar M.O., Aldurdunji M.M., Elhady S.S., Ahmad T.A, & Said A.M. (2023). Integration of immunoinformatics and cheminformatics to design and evaluate a multitope vaccine against Klebsiella pneumoniae and Pseudomonas aeruginosa coinfection. Frontiers in Molecular Biosciences, 10, 1123411.
Publication 2023
AdaptationCodon E coli Gene Organism k Strain Vaccine
Corresponding Organization : Sinai University
Other organizations :
Zagazig University, Egyptian Government, Suez Canal University, King Abdulaziz University, Umm al-Qura University, Bibliotheca Alexandrina
Codon optimization for the designed potential vaccine
dependent variables
Codon adaptation index (CAI) for the constructed potential vaccine to be expressed in E. coli k-12
control variables
E. coli k-12 strain as the expression organism
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