A total of 287 markers were selected from previous studies (Lowe et al., 2002 (link); Wang et al., 2008 (link); Ban, 2009 ; Cheng et al., 2009 (link); Li, 2010 ; Liu et al., 2014 (link); Song et al., 2015 (link); Chen et al., 2017 (link); Liu, 2017 ; Li et al., 2018 (link); He et al., 2021 (link)) (Table S3). The selected SSR markers were labelled with 6-FAM (6-carboxyfluorescein), HEX (hexachlorofluorescein), ROX (6-carboxyl-X-rhodamine; passive reference dye), and TAMRA (5-carboxytetramethylrhodamine) fluorescent dyes at the 5′ end of the forward primer. The total volume of the polymerase chain reaction (PCR) was 20 µL, with a dNTP concentration of 0.20 mmol/L, and concentrations of forward and reverse primers of 0.25 µmol/L, 0.05 U/µL of Taq total genomic DNA polymerase, 1 × PCR buffer (containing Mg2+, 2.5 mmol/l), and 50 ng/µL of DNA, and with the addition of double-distilled H2O up to a total of 20 µL. The PCR reaction conditions were as follows: pre-denaturation at 94°C for 5 minutes; denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds, extension at 72°C for 45 seconds, for a total of 35 cycles; followed by extension at 72°C for 10 minutes; and then storage of the PCR reaction at 4°C.
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