The animals were subjected to an anesthetic procedure with 2% xylazine hydrochloride (Xilazin®, Syntec, Santana de Parnaíba, Brazil) (10 mg·Kg−1) associated with 5% dextrocetamine hydrochloride (Ketamin®, Cristália, Itapira, Brazil) (25 mg·Kg−1), prepared by combining 0.5 mL of xylazine (10 mg) with 0.5 mL of ketamine (25 mg) to a volume of 1.0 mL, which was administered intraperitoneally (1.0 mL·Kg−1).
After being anesthetized, the respective animals were positioned on appropriate tables in the horizontal prone position, and skin antisepsis with chlorhexidine 2% (RIOHEX®, Rioqumica, So José do Rio Preto, Brazil), followed by 0.5% alcoholic chlorhexidine (RIOHEX®, Rioqumica, So José do Rio Preto, Brazil), was performed as part of the pre-surgical preparation [132 (link)]. With the help of a scalpel (handle and blade number 15), Mezenbaum scissors curve, and anatomical forceps, each animal—having already been identified by its group—had one circular excision of the skin made in the median plane of the dorsal region, which was constrained in depth by the muscular aponeurosis. This was accomplished by precisely measuring the 2 cm diameter of each excision using a caliper and a plastic circular mold (Universal Digimess 100003) [132 (link)]. Thereafter, animals were housed individually and monitored in properly disinfected cages to prevent infection or further damage to the wounds after recovering from anesthesia.
After being anesthetized, the respective animals were positioned on appropriate tables in the horizontal prone position, and skin antisepsis with chlorhexidine 2% (RIOHEX®, Rioqumica, So José do Rio Preto, Brazil), followed by 0.5% alcoholic chlorhexidine (RIOHEX®, Rioqumica, So José do Rio Preto, Brazil), was performed as part of the pre-surgical preparation [132 (link)]. With the help of a scalpel (handle and blade number 15), Mezenbaum scissors curve, and anatomical forceps, each animal—having already been identified by its group—had one circular excision of the skin made in the median plane of the dorsal region, which was constrained in depth by the muscular aponeurosis. This was accomplished by precisely measuring the 2 cm diameter of each excision using a caliper and a plastic circular mold (Universal Digimess 100003) [132 (link)]. Thereafter, animals were housed individually and monitored in properly disinfected cages to prevent infection or further damage to the wounds after recovering from anesthesia.
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