To evaluate the effect of decellularization on pulmonary valves, a scanning electron microscope (SEM) was used [33 ]. One sample from the pulmonary wall was collected at each step of the procedure (after Tergitol, sodium cholate, Benzonase® and one after cell seeding) and compared to the native tissue. Photographs of each patch were taken at 200×, 3000×, and 8000× magnification.
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