Lymphoma cells were collected, attached to glass slides using the cytospin method, and processed for immunofluorescence as previously reported [4 (link), 29 (link)]. To evaluate polyglutamylation levels in cells, anti-polyglutamylation antibodies (AdipoGen) were used at 1:2000 dilution. 4′,6-Diamidino-2-phenylindole (DAPI; FluoroPure grade; Thermo Fisher Scientific, Waltham, MA, USA) was used for counterstaining.
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