Immunohistochemical Profiling of Breast Markers

Immunohistochemical staining of TMA section was performed as previously described^{49 (link)}. Rabbit polyclonal antibodies for ERα (BS1114), ERβ (BS8465), PR (BS1766) and EGFR (BS1533) were purchased from Bioworld Technology (Minnesota, USA). Rabbit polyclonal antibody for ERα36 (CY1109) was purchased from Cell Applications (San Diego, CA, USA). Rabbit polyclonal antibody for HER2 (ab131490) was purchased from Abcam (Cambridge, MA, USA). These rabbit polyclonal antibodies were used as primary antibodies at 1:100 dilution and biotinylated goat-anti-rabbit IgG (ZB-2010, Zhongshan Golden Bridge Biotechnology, China) was used as a secondary antibody at 1:500 dilution.

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Dai Y.J., Qiu Y.B., Jiang R., Xu M., Zhao L., Chen G.G, & Liu Z.M. (2017). Concomitant high expression of ERα36, EGFR and HER2 is associated with aggressive behaviors of papillary thyroid carcinomas. Scientific Reports, 7, 12279.

Publication 2017

CY1109 ab131490

Anti igg Antibodies Antibody Cell Dilution Egfr Goat Her2 Rabbit

Corresponding Organization :

Other organizations : Chongqing Medical University, Chinese University of Hong Kong, Prince of Wales Hospital

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Variable analysis

independent variables

Antibody type (ERα, ERβ, PR, EGFR, ERα36, HER2)

dependent variables

Immunohistochemical staining of TMA section

control variables

Dilution of primary antibodies (1:100)
Dilution of secondary antibody (1:500)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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