E. histolytica strain HM-1:IMSS trophozoites were maintained in 50 ml culture flasks (Greiner Bio-One) containing TYI-S-33 media, 10% (v/v) heat-inactivated adult bovine serum (Sigma), 1% (v/v) MEM Vitamin Solution (Gibco), supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL) (Omega Scientific) [20 (link)]. E. invadens strain IP-1 were cultured in LYI-S-2 at 25°C [32 (link), 33 ].
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