Protein was extracted by the RIPA buffer and subjected to the regular western procedure9 (link). The following antibodies were used for western blotting: anti-UCP1 (1:1000 for WAT, 1:10000 for BAT, Abcam, ab209483), anti-BDKRB2 (1:1000, ABclonal, A2844), anti-PREP (1:1000, A9838, ABclonal), anti-HMWK (1:500, sc-23914, Santa Cruz), anti-β-Actin (1:3000, 66009-1-Ig, Proteintech), anti-N-Cadherin (1:1000; A19083; ABclonal).
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