Six male ISA Brown chickens were infected orally at day of hatch with 107 CFU of wild-type Salmonella Enteritidis 147 [15 (link)] or its isogenic SPI1 mutant [16 (link)], and sacrificed 4 days later. Six non-inoculated 5-day-old chickens were included as a control group. Approx. 30 mg of the cecum was collected from each chicken during necropsy, immediately placed into RNAlater (Qiagen) and stored at −80 °C.
In the second experiment, 64 male ISA Brown chickens were infected orally at day of hatch with 107 CFU of wild-type Salmonella Enteritidis 147 and sacrificed on day 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 18, 22, 25 and 29 of life, 4 chickens each day. Sixty-eight non-infected chickens were included as controls; four non-infected chickens were sacrificed on day 1 and the remaining at the same time points as the infected ones. During necropsy, approx. 30 mg of the cecum was collected into RNAlater (Qiagen) and stored at −80 °C.
In the second experiment, 64 male ISA Brown chickens were infected orally at day of hatch with 107 CFU of wild-type Salmonella Enteritidis 147 and sacrificed on day 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 18, 22, 25 and 29 of life, 4 chickens each day. Sixty-eight non-infected chickens were included as controls; four non-infected chickens were sacrificed on day 1 and the remaining at the same time points as the infected ones. During necropsy, approx. 30 mg of the cecum was collected into RNAlater (Qiagen) and stored at −80 °C.
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