Densitometric analysis of TH-positive fibers in the striatum was performed as previously described [37 (link)]. An average of 8 sections from the striatum (bregma +1.18 mm to +0.02 mm; anteroposterior axis) were examined using Image-Pro Plus (vision 6.0, Media Cybernetics) on a computer attached to a light microscope (Leica, Germany).
To measure the density of TH-positive cells in the SNpc we performed stereological cell counting as previously described [19 (link), 37 (link)]. Total numbers of TH-positive neurons and Nissl-stained neurons in the SNpc were counted using the optical fractionator method on a Stereo Investigator system (Micro Brightfield, USA) attached to a Leica microscope. A total of 8 sections (one every five 30 μm-thick sections collected −2.80 to −3.80 mm from bregma) were analyzed. Assays were performed in a double-blind fashion by two operators.
To measure the density of TH-positive cells in the SNpc we performed stereological cell counting as previously described [19 (link), 37 (link)]. Total numbers of TH-positive neurons and Nissl-stained neurons in the SNpc were counted using the optical fractionator method on a Stereo Investigator system (Micro Brightfield, USA) attached to a Leica microscope. A total of 8 sections (one every five 30 μm-thick sections collected −2.80 to −3.80 mm from bregma) were analyzed. Assays were performed in a double-blind fashion by two operators.
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