Whole Cell Protein Extraction and Quantification
Corresponding Organization : University of Tasmania
Other organizations : Baker Heart and Diabetes Institute
Variable analysis
- Whole cell protein lysates preparation in RIPA Lysis buffer in the presence of protease and protein phosphatase inhibitors
- Protein concentrations of the supernatants
- Vortexing of whole cell lysates every 10 min for 1 h on ice
- Centrifugation of whole cell lysates at 15,000 rpm for 5 min
- No positive or negative controls were explicitly mentioned.
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