Histological Analysis of Liver, Pancreas, and Adipose Tissue

Samples of the liver, pancreas, and epididymal adipose tissue were fixed with 10% formalin solution. After fixation, the specimens were dehydrated, embedded in paraffin, cut in a microtome to a thickness of 5 mm each, and stained with hematoxylin-eosin. An expert pathologist performed the histological analysis of the liver and pancreas. For the analysis of treatment effects on the hepatocytes, a scoring system was used [32 (link)]. In the evaluation of the architecture of the pancreas, there were changes in the Islets of Langerhans and pancreatic acini, and inflammation was observed [33 (link),34 (link)]. For the analysis of the adipocyte area of the epididymal adipose tissue, the images were initially taken using a LEICA DFC 495 digital camera system (Leica Microsystems, Wetzlar, Germany) integrated into a LEICA DM 5500B microscope (Leica Microsystems, Wetzlar, Germany), with a magnification of 20X. The images were analyzed using the LEICA Application Suite software, version 4.0 (Leica Microsystems, Wetzlar, Germany), and the mean area of 100 adipocytes per sample was determined [35 (link)].