Serum Lipid Biomarker Analysis

Venous blood samples were obtained from all subjects after at least 12 h of fasting. The levels of serum TC, TG, HDL-C, and LDL-C in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (Randox Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim BT29 4QY, U.K., ), Cholestest N HDL and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay (Randox Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [33 (link)–36 (link)].

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Zhang Q.H., Yin R.X., Chen W.X., Cao X.L, & Chen Y.M. (2018). Association between the TIMD4-HAVCR1 variants and serum lipid levels, coronary heart disease and ischemic stroke risk and atorvastatin lipid-lowering efficacy. Bioscience Reports, 38(1), BSR20171058.

Publication 2018

Tcho-1 TG-LH immunoturbidimetric immunoassay Type 7170A

Apoa1 Apob Diamond Enzymatic Immunoassay Immunoturbidimetric Randox Serum Venous blood

Corresponding Organization : Guangxi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum total cholesterol (TC)
Serum triglycerides (TG)
Serum high-density lipoprotein cholesterol (HDL-C)
Serum low-density lipoprotein cholesterol (LDL-C)
Serum apolipoprotein A1 (ApoA1)
Serum apolipoprotein B (ApoB)

control variables

Fasting for at least 12 hours before blood sample collection

controls

No positive or negative controls were explicitly mentioned.

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