CFPAC-1 Organoid Culture Protocol

Organoids were established as described before (30 (link)). CFPAC-1 cells were embedded in growth factor-reduced Matrigel (Cat#356231; BD Bioscience, CA, USA), and cultured in human complete medium at 37 C° for 14 days (21 (link), 25 (link)). Human complete medium was AdDMEM/F12(Cat#12634–010; Invitrogen, CA, USA), medium supplemented with 1M HEPES (Invitrogen), GlutaMax (Cat#35050–061; Invitrogen), penicillin/streptomycin (Cat#15140122; Invitrogen), B27 (Cat#17504044; Invitrogen), N-acetyl-l-cysteine (Cat#A9165; Sigma-Aldrich Co.), Wnt-3a (Cat#5036-WN-010; R&D Systems, MN, USA), R-Spondin1 (Cat#120–38; Peprotech, NJ, USA), Noggin (Cat#120-10C; Invitrogen), epidermal growth factor (EGF, Cat#AF-100-15; Peprotech), fibroblast growth factor (FGF, Cat#100–26; Peprotech), nicotinamide (Cat#N0636; Sigma-Aldrich Co.), Y-27263 (Cat# Y0503; Sigma-Aldrich Co.) and A83–01 (Cat#2939/10; R&D Systems).

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Yan Z., Qu J., Li Z., Yi J., Su Y., Lin Q., Yu G., Lin Z., Yin W., Lu F, & Liu J. (2021). NEK7 Promotes Pancreatic Cancer Progression And Its Expression Is Correlated With Poor Prognosis. Frontiers in Oncology, 11, 705797.

Publication 2021

AdDMEM/F12 HEPES GlutaMax penicillin/streptomycin N-acetyl-l-cysteine Wnt-3a R-Spondin1 Noggin EGF FGF nicotinamide Y-27263 A83–01

A83 01 Cells Epidermal growth factor Growth factor Hepes Human Matrigel N acetyl l cysteine Nicotinamide Noggin Organoids Penicillin Streptomycin

Corresponding Organization :

Other organizations : Peking University Shenzhen Hospital, University of Hong Kong - Shenzhen Hospital, Peking University

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Variable analysis

independent variables

CFPAC-1 cells were embedded in growth factor-reduced Matrigel
CFPAC-1 cells were cultured in human complete medium

dependent variables

Organoid formation and growth

control variables

Organoids were established as described before (30)
CFPAC-1 cells were cultured at 37 C° for 14 days

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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