The endpoints of the study were markers of oxidative balance/stress and endothelial dysfunction, which have important pathophysiologic and prognostic roles in atherothrombosis. Thus, through an acute in vivo study6 (link),7 (link), precise and informative insights were provided based on: 1) serum levels of soluble NOX2-derived peptide (sNOX2-dp), 2) serum levels of nitric oxide (NO) bioavailability, 3) serum levels of 8-iso-prostaglandin F2α-III (8-iso-PGF2α-III), 4) serum levels of vitamin E, and 5) brachial FMD.
Specifically, sNOX-2-dp was measured using an enzyme-linked immunosorbent assay as described by Loffredo et al8 (link). Nitric oxide bioavailability was measured with a colorimetric assay kit (Abcam, DRG International), 8-iso-PGF2α-III was appraised with a colorimetric assay kit (Abcam, DRG International), and vitamin E was analyzed using an Agilent 1200 Infinity series high-performance liquid chromatography system (Agilent Technologies, equipped with an Eclipse Plus C18 column), with results presented as the ratio between the concentration of a-tocopherol and serum total cholesterol. Brachial FMD was measured with a standardized and validated procedure8.
Specifically, sNOX-2-dp was measured using an enzyme-linked immunosorbent assay as described by Loffredo et al8 (link). Nitric oxide bioavailability was measured with a colorimetric assay kit (Abcam, DRG International), 8-iso-PGF2α-III was appraised with a colorimetric assay kit (Abcam, DRG International), and vitamin E was analyzed using an Agilent 1200 Infinity series high-performance liquid chromatography system (Agilent Technologies, equipped with an Eclipse Plus C18 column), with results presented as the ratio between the concentration of a-tocopherol and serum total cholesterol. Brachial FMD was measured with a standardized and validated procedure8.
Free full text: Click here
