Ectopic Angiogenesis Analysis in Zebrafish

mttp^stl/stl, mttp^c655/c655 and WT zebrafish in the Tg(fli1:eGFP)^y1 background were imaged at 3 dpf with a Zeiss Axiozoom V16 microscope equipped with a Zeiss PlanNeoFluar Z 1x/0.25 FWD 56 mm objective, AxioCam MRm camera, and Zen 2.5 software. The length of ectopic angiogenic segments that extend from the subintestinal vessels were analyzed in Fiji [104 (link)](ImageJ V2.0.0, National Institutes of Health (NIH), USA) as described by [49 (link)].

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Wilson M.H., Rajan S., Danoff A., White R.J., Hensley M.R., Quinlivan V.H., Recacha R., Thierer J.H., Tan F.J., Busch-Nentwich E.M., Ruddock L., Hussain M.M, & Farber S.A. (2020). A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein. PLoS Genetics, 16(8), e1008941.

Publication 2020

Axiozoom V16 microscope PlanNeoFluar Z 1x/0.25 FWD 56 mm objective AxioCam MRm camera Zen 2.5 software

Angiogenic Microscope Vessels Zebrafish

Corresponding Organization : University of Oulu

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Variable analysis

independent variables

Mttp^stl/stl
Mttp^c655/c655
WT zebrafish in the Tg(fli1:eGFP)^y1 background

dependent variables

Length of ectopic angiogenic segments that extend from the subintestinal vessels

control variables

Imaging of samples at 3 dpf with a Zeiss Axiozoom V16 microscope equipped with a Zeiss PlanNeoFluar Z 1x/0.25 FWD 56 mm objective, AxioCam MRm camera, and Zen 2.5 software
Analysis of the length of ectopic angiogenic segments in Fiji [104 (link)] (ImageJ V2.0.0, National Institutes of Health (NIH), USA) as described by [49 (link)]

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