In Vivo Optical Imaging of HA Hydrogel and MSCs

Serial in vivo optical imaging was performed to assess the localization of the HA hydrogel compound and the MSCs. MSCs were tagged with Qtracker^TM (Invitrogen, Eugene, OR, USA, Cat#: Q25061MP), which enabled in vivo imaging of live cells, and HA hydrogels were tagged by covalently bonding the near-infrared marker Cy7.5 to the Ad-HA component of the HA hydrogel as previously described [8 (link),9 (link)]. The animals were imaged utilizing the Pearl^® imaging system (Li-Cor, Lincoln, NE, USA) prior to injection, immediately postinjection and on days 3–7, 14, 21, and 28. Signal intensity measured in photons/pixel/second was analyzed (Pearl Impulse Software v2.0), normalized per animal to peak intensity, and averaged per cohort.

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Han D.S., Erickson C., Hansen K.C., Kirkbride-Romeo L., He Z., Rodell C.B, & Soranno D.E. (2023). Mesenchymal Stem Cells Delivered Locally to Ischemia-Reperfused Kidneys via Injectable Hyaluronic Acid Hydrogels Decrease Extracellular Matrix Remodeling 1 Month after Injury in Male Mice. Cells, 12(13), 1771.

Publication 2023

Pearl® imaging system

Animal Cells Hydrogel

Corresponding Organization : Purdue University West Lafayette

Other organizations : Stanford University, University of Colorado Anschutz Medical Campus, University of Colorado Denver, Drexel University

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Variable analysis

independent variables

HA hydrogel compound

dependent variables

Localization of the HA hydrogel compound and the MSCs

control variables

Qtracker(TM) used to tag the MSCs
Cy7.5 used to tag the HA hydrogels

controls

No positive or negative controls were explicitly mentioned in the provided information.

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