The immunofluorescence staining was performed on OE19 and JHESO EAC cells treated with PVT1 ASO at 1 μM as performed as previously described [18 (link)]. Expression and localization of the proteins were observed under a confocal microscope system (FluoView FV500; Olympus) and analyzed by CellQuest PRO software (BD Biosciences, San Jose, CA). Information for antibodies used in immunofluorescent staining and western blot was described in Additional file 6: Figure S6.
Xu Y., Li Y., Jin J., Han G., Sun C., Pizzi M.P., Huo L., Scott A., Wang Y., Ma L., Lee J.H., Bhutani M.S., Weston B., Vellano C., Yang L., Lin C., Kim Y., MacLeod A.R., Wang L., Wang Z., Song S, & Ajani J.A. (2019). LncRNA PVT1 up-regulation is a poor prognosticator and serves as a therapeutic target in esophageal adenocarcinoma. Molecular Cancer, 18, 141.
Other organizations :
The University of Texas MD Anderson Cancer Center, Ionis Pharmaceuticals (United States), China Medical University, First Hospital of China Medical University
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