Immunoblotting Reagents for Protein Detection

The rabbit polyclonal anti-Myc antibody and the biotin was purchased from Sigma-Aldrich. The mouse anti-EF1α antibody was purchased from Santa Cruz Biotechnology. The mouse monoclonal anti-TAC102 antibody and the rabbit anti-ATOM antibody have been described previously [24 (link),48 (link)]. Streptavidin-conjugated magnetic beads were purchased from NEB. HRP-conjugated streptavidin and AlexaFluor488-conjugated streptavidin were purchased from Thermo Fisher Scientific.

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Baudouin H.C., Pfeiffer L, & Ochsenreiter T. (2020). A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. PLoS Neglected Tropical Diseases, 14(9), e0008568.

Publication 2020

anti-Myc antibody biotin Streptavidin-conjugated magnetic beads HRP-conjugated streptavidin AlexaFluor488-conjugated streptavidin

Anti antibody Biotin Monoclonal antibody Mouse Rabbit Streptavidin

Corresponding Organization : University of Bern

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal anti-Myc antibody
Biotin

dependent variables

Unspecified

control variables

Mouse anti-EF1α antibody
Mouse monoclonal anti-TAC102 antibody
Rabbit anti-ATOM antibody
Streptavidin-conjugated magnetic beads
HRP-conjugated streptavidin
AlexaFluor488-conjugated streptavidin

positive controls

Mouse anti-EF1α antibody
Mouse monoclonal anti-TAC102 antibody

negative controls

Unspecified

Annotations

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