Assessing C12O3TR Impact on P. digitatum Cell Wall

The effect of C₁₂O₃TR on P. digitatum cell wall integrity was determined as described by OuYang et al. (2019) (link), with some modifications. P. digitatum conidia (1 × 10⁴ CFU/mL, 90 μL) mixed with 5% PDB were cultured at 25°C for 48 h. C₁₂O₃TR with the final concentrations of 6.25 μmol/L (MIC) was added and then incubated for 0, 2, and 12 h. PBS (pH 7.0) was used as control. Each sample was then stained with 50 mg/L Calcofluor White (CFW) for 5 min in dark, and the fluorescence was examined and photographed by the Eclipse TS100 epifluorescence microscope (Nikon Corporation, Japan) with DAPI filter sets. The reproducibility of experiment results was confirmed by three replicates.