Neonatal IgG Transfer from Maternal to Cord Blood

The study was approved by the cantonal Ethics Committee of Zurich (KEK Nr. 2019-00150). All experiments were performed in accordance with relevant guidelines and regulations. Informed consent was obtained from all participants and/or their legal guardians. Leftover maternal and cord blood samples were obtained from the University hospital Zurich, Switzerland. The study population comprised 30 full-term and 12 pre-term neonates. Out of these 42 samples, matched maternal blood was available for 26 full-term and 5 pre-term cord blood samples. Blood was centrifuged at 3,000 x g for 15 min at 4°C and sera were frozen at −20°C. IgG and IgM concentrations were determined by ELISA [IgG human ELISA Kit from Abnova (KA3817), IgM human ELISA Kit from Abnova (KA1855), Taipeh, China] according to the manufacturer's protocols. IgG transfer was calculated as proportion of the IgG concentration in cord blood to the IgG concentration in maternal blood ([IgG_cord/IgG_maternal]^*100), according to (44 (link)).