Plasma Gd-IgA1 levels were detected using ELISA kit according to the manufacturer’s specifications (IBL, Japan) [6 (link)]. Plasma samples were diluted in proportions of 1:50 with EIA buffer and incubation for 60 min at R.T. with plate lid. Then washing four times with wash buffer prepared labeled antibody were incubated for 30 min. Plates were washed and added 50ul TMA solution incubation for 30 min in dark. At last, the color reaction was stopped and the absorbance was measured at 450/625 nm with an EL312 Bio-Kinetics microplate reader (Bio-TekInstruments, Winooski, VT).
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