Western Blot Antibody Sources

Goat anti-HSP27 (M-20), and mouse anti-αA-crystallin (B-2) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit anti-vimentin, rabbit anti-histone H3, rabbit anti-MEK1/2, rabbit anti-HSP40, rabbit anti-HSP60, rabbit anti-HSP70, rabbit anti-HSP90, rabbit anti-AIF, goat anti-rabbit IgG-HRP-linked and horse anti-mouse IgG-HRP-linked antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-FLAG (M2) and mouse anti-β-actin antibodies were obtained from Millipore Sigma (St. Louis, MO, USA). Rabbit αB-crystallin antibody was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Affinity purified anti-FAIM antibody was obtained from rabbits immunized with CYIKAVSSRKRKEGIIHTLI peptide (located near the C-terminal region of FAIM) as previously described [33 (link),46 (link)].

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Kaku H., Balaj A.R, & Rothstein T.L. (2022). Small Heat Shock Proteins Collaborate with FAIM to Prevent Accumulation of Misfolded Protein Aggregates. International Journal of Molecular Sciences, 23(19), 11841.

Publication 2022

Rabbit anti-vimentin rabbit anti-histone H3 rabbit anti-MEK1/2 rabbit anti-HSP60 rabbit anti-HSP70 rabbit anti-HSP90 rabbit anti-AIF goat anti-rabbit IgG-HRP-linked horse anti-mouse IgG-HRP-linked Mouse anti-FLAG (M2) mouse anti-β-actin

Actin Anti antibodies Anti antibody Anti igg Antibodies Antibody Crystallin Goat Histone h3 Horse Hsp27 Hsp40 Hsp70 Hsp90 Igg hrp Mek1 Mouse Peptide Rabbit Rabbits Vimentin

Corresponding Organization : Stryker (United States)

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Variable analysis

independent variables

Goat anti-HSP27 (M-20) antibody
Mouse anti-αA-crystallin (B-2) antibody
Rabbit anti-vimentin antibody
Rabbit anti-histone H3 antibody
Rabbit anti-MEK1/2 antibody
Rabbit anti-HSP40 antibody
Rabbit anti-HSP60 antibody
Rabbit anti-HSP70 antibody
Rabbit anti-HSP90 antibody
Rabbit anti-AIF antibody
Goat anti-rabbit IgG-HRP-linked antibody
Horse anti-mouse IgG-HRP-linked antibody
Mouse anti-FLAG (M2) antibody
Mouse anti-β-actin antibody
Rabbit αB-crystallin antibody
Affinity purified anti-FAIM antibody

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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