Mice intestinal pieces
(2–4 cm) from vehicle, NSGM, or FUOX-treated animals were subjected
to chelation and dissociation into crypts using previously described
methods.22 (link) Approximately 200 to 500 crypts
were resuspended in 100 μL of Matrigel per well of a 96-well
plate and overlaid with 100 μL of IntestiCult Organoid Growth
Medium (Mouse; Stem Cell Technologies) after allowing for Matrigel
polymerization and cultured in a CO2 incubator (37 °C,
5% CO2). The intestinal organoids were counted with a phase
contrast microscope after 5 to 7 days of plating.
(2–4 cm) from vehicle, NSGM, or FUOX-treated animals were subjected
to chelation and dissociation into crypts using previously described
methods.22 (link) Approximately 200 to 500 crypts
were resuspended in 100 μL of Matrigel per well of a 96-well
plate and overlaid with 100 μL of IntestiCult Organoid Growth
Medium (Mouse; Stem Cell Technologies) after allowing for Matrigel
polymerization and cultured in a CO2 incubator (37 °C,
5% CO2). The intestinal organoids were counted with a phase
contrast microscope after 5 to 7 days of plating.
