Male WT and Mdr2−/− mice (8 weeks of age) were implanted with osmotic minipumps to deliver 0.9% NaCl (saline) or 10 mg/kg BW/day (18 (link), 19 (link)) of either mepyramine (H1HR antagonist), ranitidine (H2HR antagonist). In separate experiments, male Mdr2−/− mice (8 weeks of age) were treated with a combination of mepyramine and ranitidine (H1HR/H2HR antagonist) for 4 weeks to mimic chronic usage. Male mice (8 – 10 mice per group) were euthanized at 12 weeks of age, where these mice display an increase in PSC-induced hepatic damage (7 , 10 (link)). From these groups, we collected liver blocks (frozen and paraffin-embedded), serum, cholangiocytes and cholangiocyte supernatants, as described (7 , 20 ).
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Histamine Receptor Antagonism in Mdr2 Mice
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Corresponding Organization : Baylor Scott & White Health
Other organizations : University of Milano-Bicocca
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Variable analysis
independent variables
- Treatment with 0.9% NaCl (saline)
- Treatment with 10 mg/kg BW/day of mepyramine (H1HR antagonist)
- Treatment with 10 mg/kg BW/day of ranitidine (H2HR antagonist)
- Treatment with a combination of mepyramine and ranitidine (H1HR/H2HR antagonist)
- Hepatic damage in Male Mdr2^-/- mice at 12 weeks of age
- Male WT and Mdr2^-/- mice (8 weeks of age)
- Positive control: Male Mdr2^-/- mice (8 weeks of age) treated with saline
- Negative control: Male WT mice (8 weeks of age) treated with saline
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