Intracellular Staining of Immune Cells

Intracellular staining was performed as described previously (40 (link)). Splenocytes and peripheral blood mononuclear cells (PBMCs) were stained with the following antibodies: PE-Cy 7-CD4 (#201516; BioLegend, San Diego, CA, USA), FITC-CD25 (#202103; BioLegend), PE-Foxp3 (#12-5773-82; eBioscience, San Diego, CA, USA), FITC-IFN-γ (#507804; BioLegend), PE-Cy5.5-IL-17A (#45-7177-82, eBioscience), and PE-IL-10 (#555088, BD Biosciences, Franklin Lakes, NJ, USA). Events were analyzed using FlowJo software (Tree Star, Ashland, OR, USA) and CytExpert experiment-based software (Beckman Coulter, Inc, Bera, CA, USA.).

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Cho K.H., Na H.S., Jhun J., Woo J.S., Lee A.R., Lee S.Y., Lee J.S., Um I.G., Kim S.J., Park S.H, & Cho M.L. (2022). Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes. Frontiers in Immunology, 13, 930511.

Publication 2022

PE-Cy 7-CD4 FITC-CD25 PE-Foxp3 FITC-IFN-γ PE-IL-10 CytExpert experiment-based software

Antibodies Cd25 Cy5 5 Fitc Ifn γ Il 10 Il 17a Intracellular Peripheral blood mononuclear cells Tree

Corresponding Organization : Catholic University of Korea

Other organizations : Seoul St. Mary's Hospital

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Variable analysis

independent variables

Intracellular staining protocol

dependent variables

Expression of CD4, CD25, Foxp3, IFN-γ, IL-17A, and IL-10 in splenocytes and peripheral blood mononuclear cells (PBMCs)

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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