Fungal Identification and Characterization Protocol

Macroscopic and microscopic characters were observed and measured as previously described. The isolates were inoculated and incubated for 7 d on the agar media Czapek yeast autolysate agar (CYA), yeast extract sucrose agar (YES), creatine sucrose agar (CREA), dichloran 18% glycerol agar (DG18), oatmeal agar (OA), and malt extract agar (MEA; Oxoid CM0059) [4 (link)]. Colony colors were referenced to numbered color codes in parentheses [66 ]. Light microscope preparations were made from one wk old colonies grown on MEA. To induce ascomata formation, colonies were incubated for more than two weeks. A Zeiss Stereo Discovery V20 dissecting microscope and a Zeiss AX10 Imager A2 light microscope, both equipped with an Axiocam 506 color camera and ZEN v.2.0 software (made in Germany), were used to capture digital images.

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Sun B., Luo C., Bills G.F., Li J., Huang P., Wang L., Jiang X, & Chen A.J. (2022). Four New Species of Aspergillus Subgenus Nidulantes from China. Journal of Fungi, 8(11), 1205.

Publication 2022

CM0059 Stereo Discovery V20 AX10 Imager A2

Agar Characters Creatine Dichloran Glycerol Light microscope Microscope Sucrose Yeast

Corresponding Organization :

Other organizations : Chinese Academy of Sciences, Guangzhou Institute of Geochemistry, Brown Foundation

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Variable analysis

independent variables

Agar media types: Czapek yeast autolysate agar (CYA), yeast extract sucrose agar (YES), creatine sucrose agar (CREA), dichloran 18% glycerol agar (DG18), oatmeal agar (OA), and malt extract agar (MEA)

dependent variables

Macroscopic and microscopic characters of the isolates
Colony colors
Ascomata formation

control variables

Incubation time: 7 days for initial inoculation and more than two weeks for ascomata formation
Microscope used: Zeiss Stereo Discovery V20 dissecting microscope and Zeiss AX10 Imager A2 light microscope, both equipped with an Axiocam 506 color camera and ZEN v.2.0 software

controls

No positive or negative controls were explicitly mentioned in the provided information.

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