Immunohistochemical Analysis of Angiogenesis in Tumor Tissues

Tissue sample preparation for immunohistochemical evaluation was carried out in the Histology and Tissue Analysis Core at Dell Pediatric Research Institute (DPRI) at The University of Texas at Austin. The formalin-fixed tumor tissues were embedded in paraffin wax, sectioned, and stained with an antibody against CD-31 (Abcam, Cambridge, MA, USA) as a marker for angiogenesis (n = 3) ^{41 (link)}. Slides were then scanned, and images were taken using the ScanScope XT (Aperio Technologies, Vista, CA, USA).

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Naguib Y.W., Rodriguez B.L., Li X., Hursting S.D., Williams RO I.I.I, & Cui Z. (2014). Solid Lipid Nanoparticle Formulations of Docetaxel Prepared with High Melting Point Triglycerides: In Vitro and in Vivo Evaluation. Molecular Pharmaceutics, 11(4), 1239-1249.

Publication 2014

antibody against CD-31 ScanScope XT

Angiogenesis Antibody Austin Formalin Paraffin wax Tissues Tumor

Corresponding Organization :

Other organizations : The University of Texas at Austin

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Variable analysis

independent variables

Tissue samples

dependent variables

Angiogenesis

control variables

Formalin-fixed tumor tissues
Paraffin wax embedding
Sectioning
Staining with anti-CD-31 antibody

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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