The following ligands were used: Pam3CSK4 (TLR1/2; tlrl-pms; Invivogen, 200 ng/ml), FSL1 (TLR2/6; tlrl-fsl; Invivogen, 100 ng/ml), Poly (I:C) (TLR3; vac-pic; Invivogen, 10 μg /ml), LPS K12 (TLR4; tlrl-eklps; Invivogen, 200 ng/ml), Flagellin (TLR5; tlrl-stfla; Invivogen, 100 ng/ml), R837 (TLR7; tlrl-imqs; Invivogen, 10 μg/ml), R848 (TLR7/8; tlrl-r848; Invivogen, 100 ng/ml), CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), and CpG2006 (TLR9; tlrl-2006; Invivogen, 10 μM), and human interferon-beta 1a (100U/ml) was purchased from PeproTech GmbH (#300–02BC; Germany), 2’3’-cGAMP (InvivoGen, 1ug/ml), Lipofectamine 3000 (Invitrogen), diABZI STING agonist-1 (MedChemExpress, 1μM), Sendai Virus (Cantell Strain) (Charles River, 10 HA units/ml), and HSV-1 (MOI = 1), infected as previously described [28 (link)].
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