Thermal Stability of SRL10 Protein

Purified recombinant proteins GST‐SRL10, His‐CATB and total proteins from 20‐d‐old seedlings of WYJ7 were used for cell‐free degradation assay, and was carried out as previously described (Bello et al., 2019 (link); Lv et al., 2014 (link)). The dilution for the mouse anti‐GST antibody (TransGen Biotech) and the mouse anti‐His antibody (TransGen Biotech) was 1:5000. Reactions were terminated at indicated time points, and the protein abundance was visualized via immune detection against anti‐His and anti‐GST. Total proteins from NIL‐SRL10^Hap1 and NIL‐SRL10^Hap3 were used for thermal stability analysis of protein and incubated under 28 °C and 42 °C for 0, 30, 60 and 90 min respectively. The dilution for the rabbit anti‐SRL10 antibody was 1:10000.

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Wang J., Xu J., Wang L., Zhou M., Nian J., Chen M., Lu X., Liu X., Wang Z., Cen J., Liu Y., Zhang Z., Zeng D., Hu J., Zhu L., Dong G., Ren D., Gao Z., Shen L., Zhang Q., Li Q., Guo L., Yu S., Qian Q, & Zhang G. (2023). SEMI‐ROLLED LEAF 10 stabilizes catalase isozyme B to regulate leaf morphology and thermotolerance in rice (Oryza sativa L.). Plant Biotechnology Journal, 21(4), 819-838.

Publication 2023

mouse anti‐GST antibody mouse anti‐His antibody

Anti antibody Antibody Assay Cell Dilution Mouse Proteins Rabbit Recombinant proteins Seedlings

Corresponding Organization : Chinese Academy of Agricultural Sciences

Other organizations : Huazhong Agricultural University, Health First, Chinese Academy of Forestry

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Variable analysis

independent variables

Purified recombinant proteins GST-SRL10 and His-CATB
Total proteins from 20-d-old seedlings of WYJ7
Incubation temperature (28 °C and 42 °C)

dependent variables

Protein abundance (visualized via immune detection against anti-His and anti-GST)
Thermal stability of SRL10 protein

control variables

Cell-free degradation assay protocol (previously described in Bello et al., 2019 and Lv et al., 2014)
Dilution of mouse anti-GST antibody (1:5000)
Dilution of mouse anti-His antibody (1:5000)
Dilution of rabbit anti-SRL10 antibody (1:10000)
Incubation time points (0, 30, 60 and 90 min)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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