Kidney Injury Biomarker Quantification

Immunohistochemical staining of NGAL, KIM-1, and Neutrophil Elastase on formalin-fixed paraffin-embedded human kidney tissue was performed as described (Supplemental Digital Content 1, http://links.lww.com/CCX/A103). To quantify NGAL and KIM-1 immunostaining, the sections were first scanned using a Nanozoomer HT (Hamamatsu Photonics, Hamamatsu, Japan). Morphometric analysis was performed using the Aperio Imagescope positive pixel analysis v9.1 algorithm (Aperio Technologies, Vista, CA) as described previously (23 (link)). Neutrophil infiltration was quantified by counting the number of neutrophils (neutrophil Elastase positive) present in all glomeruli of the kidney sections.

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Jou-Valencia D., Koeze J., Popa E.R., Aslan A., Zwiers P.J., Molema G., Zijlstra J.G., van Meurs M, & Moser J. (2019). Heterogenous Renal Injury Biomarker Production Reveals Human Sepsis-Associated Acute Kidney Injury Subtypes. Critical Care Explorations, 1(10), e0047.

Publication 2019

Nanozoomer HT Imagescope positive pixel analysis v9.1 algorithm

Digital 1 Embedded paraffin Formalin Glomeruli kidney Human Kidney Kim 1 Neutrophil elastase Neutrophil infiltration Neutrophils Ngal

Corresponding Organization :

Other organizations : University Medical Center Groningen, University of Groningen

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Variable analysis

independent variables

Immunohistochemical staining of NGAL, KIM-1, and Neutrophil Elastase on formalin-fixed paraffin-embedded human kidney tissue

dependent variables

NGAL immunostaining
KIM-1 immunostaining
Neutrophil infiltration (number of neutrophils present in all glomeruli of the kidney sections)

control variables

Formalin-fixed paraffin-embedded human kidney tissue

controls

No positive or negative controls were explicitly mentioned in the provided information.

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