Using the databases TargetScan (http://targetscan.org/), PicTar (http://pictar.mdc-berlin.de/) and miRDB (http://www.mirdb.org/miRDB/), the putative target genes of miR-30c were investigated, and the ASF/SF2 gene was identified among them. To amplify the 3′UTR sequence of the human ASF/SF2 gene, primers were designed. Mlu1 and Spe1 sequences were cloned into the ends of primers (Promega Corporation, Madison, WI, USA). According to the manufacturer's protocol, 3′-UTR cloning was performed in a pMIR reporter vector (Promega Corporation). The pMIR-ASF/SF2 3′-UTR reporter vector (100 ng) was cotransfected with 50 ng of β-Gal and miRNA-30c mimic or anti-miR-30c using Lipofectamine 2000 transfection reagent. All Stars negative control siRNA (Qiagen, Inc.) and miScript inhibitor negative control (Qiagen, Inc.) were used as controls for mimic or inhibitor transfection, respectively. At 24 h post-transfection, luciferase reporter assays were performed, as described previously (9 (link)).