Nanoparticle Size Characterization via PCCS

Dynamic light scattering using photon cross-correlation spectroscopy (PCCS) (NanoPhox, Sympatec, Germany) was used to determine the hydrodynamic size of the NPs (10 μg/mL) in cell medium. In brief, 1 mL dispersions of Ni and NiO (10 μg/mL) were prepared in cuvettes and analyzed directly after dispersion (0 h), after 2 and 24 h. Triplicate samples were analyzed to identify the size distribution pattern and data is presented based on single sample analysis measured three times each. Calibration was made using standard latex samples (20 ± 2 nm) and blank samples were analyzed prior to all measurements [28 (link)].