Construction of Recombinant Ad Vectors Expressing HER3
The human HER3 complementary DNA (cDNA) was excised from a pCMVSport6-HER3-HsIMAGE6147464 plasmid (cDNA clone MGC:88033/IMAGE:6147464 obtained from ATCC). Construction of a first-generation (E1-, E3-) Ad vector containing human full length HER3 under control of human cytomegalovirus (CMV) promoter/enhancer elements was performed using the pAdEasy system (Agilent technologies, Santa Clara, CA, USA) as previously described [22 –24 (link)]. Similar Ad-vectors containing the green fluorescence protein (GFP) or lacZ rather than HER3 was similarly generated to serve as controls.
Osada T., Hartman Z.C., Wei J., Lei G., Hobeika A.C., Gwin W.R., Diniz M.A., Spector N., Clay T.M., Chen W., Morse M.A, & Lyerly H.K. (2018). Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers. Breast Cancer Research : BCR, 20, 90.
Cdna CloneCytomegalovirus Enhancer elements Green fluorescence protein Her3 Human Human cytomegalovirus Lacz Plasmid Vectors
Corresponding Organization :
Other organizations :
Duke University Hospital, Duke Medical Center, University of Washington Medical Center, Cedars-Sinai Medical Center, GlaxoSmithKline (United States)
Presence of human full length HER3 cDNA under control of human cytomegalovirus (CMV) promoter/enhancer elements
dependent variables
Not explicitly mentioned
control variables
Presence of green fluorescence protein (GFP) or lacZ rather than HER3 in the Ad-vectors
controls
Positive control: Ad-vectors containing GFP or lacZ
Negative control: Not mentioned
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