Quantifying PFOS in Exposure Tanks

For quantification of PFOS in exposure tanks, 1 mL of water sample was collected from each fish tank shortly after exposure (day 0) and before renewing with fresh treatment water at day 5. Samples were stored at 4 °C, and PFOS were measured by combined liquid (Agilent 1200, American) mass spectrometry (Bruker Esquire HCT ion trap, Germany) (LC/MS) according to our previous methods44 (link)45 (link). Three independent replicates of each sample were prepared and analyzed. The concentrations of PFOS were calculated from standard curves and the average extraction efficiency of PFOS ranged from 75% to 80%.

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Chen Y., Hu W., Huang C., Hua S., Wei Q., Bai C., Chen J., Norris M.B., Winn R., Yang D, & Dong Q. (2016). Subchronic perfluorooctanesulfonate (PFOS) exposure induces elevated mutant frequency in an in vivo λ transgenic medaka mutation assay. Scientific Reports, 6, 38466.

Publication 2016

Agilent 1200 HCT ion trap

Fish Mass spectrometry

Corresponding Organization :

Other organizations : Wenzhou Medical University, University of Georgia

Top 5 similar protocols

Variable analysis

independent variables

PFOS exposure concentration

dependent variables

PFOS concentration in water samples

control variables

Water sample volume (1 mL)
Water sample storage temperature (4 °C)
PFOS measurement method (LC/MS)
Number of independent replicates (3)
PFOS extraction efficiency (75% to 80%)

controls

Positive control: None mentioned
Negative control: None mentioned

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